COII ”long fragment” reliability in characterisation and classification of forensically important flies

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Reliability of long vs short COI markers in identification of forensically important flies

AIM To compare the reliability of short and long cytochrome oxidase I gene fragment (COI) in identification of forensically important Diptera from Egypt and China. METHODS We analyzed 50 specimens belonging to 18 species. The two investigated markers were amplified by polymerase chain reaction (PCR) followed by direct sequencing. Nucleotide sequence divergences were calculated using the Kimur...

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Molecular Species Identification of Forensically Important Flies in Korea

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Antennal sensilla of some forensically important flies in families Calliphoridae, Sarcophagidae and Muscidae.

Antennal sensilla of some forensically important fly species in the families Calliphoridae (Chrysomya megacephala, Chrysomya rufifacies, Chrysomya nigripes and Lucilia cuprina), Sarcophagidae (Parasarcophaga dux) and Muscidae (Musca domestica) were studied using scanning electron microscopy. Five types of sensilla were observed: trichoid, basiconic, coeloconic, styloconic and sensory pit. Only ...

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Temperature Requirements of Some Common Forensically Important Blow and Flesh Flies (Diptera) under Laboratory Conditions

BACKGROUND The aim of his study was to determine development time and thermal requirements of three myiasis flies including Chrysomya albiceps, Lucilia sericata, and Sarcophaga sp. METHODS Rate of development (ROD) and accumulated degree day (ADD) of three important forensic flies in Iran, Chrysomya albiceps, Lucilia sericata, and Sarcophaga sp. by rearing individuals under a single constant ...

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DNA-barcoding of forensically important blow flies (Diptera: Calliphoridae) in the Caribbean Region

Correct identification of forensically important insects, such as flies in the family Calliphoridae, is a crucial step for them to be used as evidence in legal investigations. Traditional identification based on morphology has been effective, but has some limitations when it comes to identifying immature stages of certain species. DNA-barcoding, using COI, has demonstrated potential for rapid a...

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ژورنال

عنوان ژورنال: Archives of Forensic Medicine and Criminology

سال: 2016

ISSN: 0324-8267

DOI: 10.5114/amsik.2016.64708